Review



40 μ m dapt  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Millipore 40 μ m dapt
    Ascl1a and Lin28a expression synergize with Notch signaling inhibition to stimulate MG proliferation throughout the uninjured retina. A, Left, mCherry expression and GS immunofluorescence (green) in uninjured retinas of Tp1:mCherry fish and indicate that Notch signaling is confined to quiescent MG. Remaining panels show that MG proliferation (BrdU+; green signal) stimulated by forced Ascl1a and Lin 28 expression in the uninjured and injured retina of hsp70:ascl1a;hsp70:lin28a;Tp1:mCherry triple-transgenic fish is accompanied by reduced mCherry expression (red). Scale bar, 50 μm. B, BrdU immunofluorescence (red) shows that forced Ascl1a and Lin28a expression synergizes with <t>DAPT-treatment</t> to stimulate MG proliferation (BrdU+) in the INL of uninjured retinas. Scale bar, 50 μm. C, Quantification of BrdU+ cells in Wt, hsp70:ascl1a, hsp70:lin28a, and hsp70:ascl1a;hsp70:lin28a fish treated with heat shock, ± DAPT <t>or</t> <t>RO4929097;</t> n = 3 different experiments. Error bars are SD. *p < 0.05, **p < 0.01, ***p < 0.001. D, BrdU immunofluorescence shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) throughout the INL of uninjured retinas. Scale bar, 150 μm. E, qPCR quantification of her4.1 gene expression in uninjured retinas treated with DMSO, DAPT, or RO4929097; n = 3 different experiments. Error bars are SD. ***p < 0.001.
    40 μ M Dapt, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/40+%CE%BC+m+dapt/5+m+dapt/pmc05830513-269-27-28
    Average 90 stars, based on 1 article reviews
    40 μ m dapt - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice"

    Article Title: Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2126-17.2018

    Ascl1a and Lin28a expression synergize with Notch signaling inhibition to stimulate MG proliferation throughout the uninjured retina. A, Left, mCherry expression and GS immunofluorescence (green) in uninjured retinas of Tp1:mCherry fish and indicate that Notch signaling is confined to quiescent MG. Remaining panels show that MG proliferation (BrdU+; green signal) stimulated by forced Ascl1a and Lin 28 expression in the uninjured and injured retina of hsp70:ascl1a;hsp70:lin28a;Tp1:mCherry triple-transgenic fish is accompanied by reduced mCherry expression (red). Scale bar, 50 μm. B, BrdU immunofluorescence (red) shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) in the INL of uninjured retinas. Scale bar, 50 μm. C, Quantification of BrdU+ cells in Wt, hsp70:ascl1a, hsp70:lin28a, and hsp70:ascl1a;hsp70:lin28a fish treated with heat shock, ± DAPT or RO4929097; n = 3 different experiments. Error bars are SD. *p < 0.05, **p < 0.01, ***p < 0.001. D, BrdU immunofluorescence shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) throughout the INL of uninjured retinas. Scale bar, 150 μm. E, qPCR quantification of her4.1 gene expression in uninjured retinas treated with DMSO, DAPT, or RO4929097; n = 3 different experiments. Error bars are SD. ***p < 0.001.
    Figure Legend Snippet: Ascl1a and Lin28a expression synergize with Notch signaling inhibition to stimulate MG proliferation throughout the uninjured retina. A, Left, mCherry expression and GS immunofluorescence (green) in uninjured retinas of Tp1:mCherry fish and indicate that Notch signaling is confined to quiescent MG. Remaining panels show that MG proliferation (BrdU+; green signal) stimulated by forced Ascl1a and Lin 28 expression in the uninjured and injured retina of hsp70:ascl1a;hsp70:lin28a;Tp1:mCherry triple-transgenic fish is accompanied by reduced mCherry expression (red). Scale bar, 50 μm. B, BrdU immunofluorescence (red) shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) in the INL of uninjured retinas. Scale bar, 50 μm. C, Quantification of BrdU+ cells in Wt, hsp70:ascl1a, hsp70:lin28a, and hsp70:ascl1a;hsp70:lin28a fish treated with heat shock, ± DAPT or RO4929097; n = 3 different experiments. Error bars are SD. *p < 0.05, **p < 0.01, ***p < 0.001. D, BrdU immunofluorescence shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) throughout the INL of uninjured retinas. Scale bar, 150 μm. E, qPCR quantification of her4.1 gene expression in uninjured retinas treated with DMSO, DAPT, or RO4929097; n = 3 different experiments. Error bars are SD. ***p < 0.001.

    Techniques Used: Expressing, Inhibition, Immunofluorescence, Transgenic Assay

    Related Articles

    Expressing:

    Article Title: Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice
    Article Snippet: For extended periods of heat shock, this was repeated every 6 h. To inhibit Notch signaling in fish, we immersed fish in water containing 40 μ m DAPT (Sigma-Aldrich) or RO4929097 (Cayman) prepared in DMSO and diluted 1/200 in fish water.

    Inhibition:

    Article Title: Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice
    Article Snippet: For extended periods of heat shock, this was repeated every 6 h. To inhibit Notch signaling in fish, we immersed fish in water containing 40 μ m DAPT (Sigma-Aldrich) or RO4929097 (Cayman) prepared in DMSO and diluted 1/200 in fish water.

    Immunofluorescence:

    Article Title: Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice
    Article Snippet: For extended periods of heat shock, this was repeated every 6 h. To inhibit Notch signaling in fish, we immersed fish in water containing 40 μ m DAPT (Sigma-Aldrich) or RO4929097 (Cayman) prepared in DMSO and diluted 1/200 in fish water.

    Transgenic Assay:

    Article Title: Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice
    Article Snippet: For extended periods of heat shock, this was repeated every 6 h. To inhibit Notch signaling in fish, we immersed fish in water containing 40 μ m DAPT (Sigma-Aldrich) or RO4929097 (Cayman) prepared in DMSO and diluted 1/200 in fish water.



    Similar Products

    40 μ m dapt  (Millipore)
    90
    Millipore 40 μ m dapt
    Ascl1a and Lin28a expression synergize with Notch signaling inhibition to stimulate MG proliferation throughout the uninjured retina. A, Left, mCherry expression and GS immunofluorescence (green) in uninjured retinas of Tp1:mCherry fish and indicate that Notch signaling is confined to quiescent MG. Remaining panels show that MG proliferation (BrdU+; green signal) stimulated by forced Ascl1a and Lin 28 expression in the uninjured and injured retina of hsp70:ascl1a;hsp70:lin28a;Tp1:mCherry triple-transgenic fish is accompanied by reduced mCherry expression (red). Scale bar, 50 μm. B, BrdU immunofluorescence (red) shows that forced Ascl1a and Lin28a expression synergizes with <t>DAPT-treatment</t> to stimulate MG proliferation (BrdU+) in the INL of uninjured retinas. Scale bar, 50 μm. C, Quantification of BrdU+ cells in Wt, hsp70:ascl1a, hsp70:lin28a, and hsp70:ascl1a;hsp70:lin28a fish treated with heat shock, ± DAPT <t>or</t> <t>RO4929097;</t> n = 3 different experiments. Error bars are SD. *p < 0.05, **p < 0.01, ***p < 0.001. D, BrdU immunofluorescence shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) throughout the INL of uninjured retinas. Scale bar, 150 μm. E, qPCR quantification of her4.1 gene expression in uninjured retinas treated with DMSO, DAPT, or RO4929097; n = 3 different experiments. Error bars are SD. ***p < 0.001.
    40 μ M Dapt, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/40+%CE%BC+m+dapt/5+m+dapt/pmc05830513-269-27-28
    Average 90 stars, based on 1 article reviews
    40 μ m dapt - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Ascl1a and Lin28a expression synergize with Notch signaling inhibition to stimulate MG proliferation throughout the uninjured retina. A, Left, mCherry expression and GS immunofluorescence (green) in uninjured retinas of Tp1:mCherry fish and indicate that Notch signaling is confined to quiescent MG. Remaining panels show that MG proliferation (BrdU+; green signal) stimulated by forced Ascl1a and Lin 28 expression in the uninjured and injured retina of hsp70:ascl1a;hsp70:lin28a;Tp1:mCherry triple-transgenic fish is accompanied by reduced mCherry expression (red). Scale bar, 50 μm. B, BrdU immunofluorescence (red) shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) in the INL of uninjured retinas. Scale bar, 50 μm. C, Quantification of BrdU+ cells in Wt, hsp70:ascl1a, hsp70:lin28a, and hsp70:ascl1a;hsp70:lin28a fish treated with heat shock, ± DAPT or RO4929097; n = 3 different experiments. Error bars are SD. *p < 0.05, **p < 0.01, ***p < 0.001. D, BrdU immunofluorescence shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) throughout the INL of uninjured retinas. Scale bar, 150 μm. E, qPCR quantification of her4.1 gene expression in uninjured retinas treated with DMSO, DAPT, or RO4929097; n = 3 different experiments. Error bars are SD. ***p < 0.001.

    Journal: The Journal of Neuroscience

    Article Title: Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice

    doi: 10.1523/JNEUROSCI.2126-17.2018

    Figure Lengend Snippet: Ascl1a and Lin28a expression synergize with Notch signaling inhibition to stimulate MG proliferation throughout the uninjured retina. A, Left, mCherry expression and GS immunofluorescence (green) in uninjured retinas of Tp1:mCherry fish and indicate that Notch signaling is confined to quiescent MG. Remaining panels show that MG proliferation (BrdU+; green signal) stimulated by forced Ascl1a and Lin 28 expression in the uninjured and injured retina of hsp70:ascl1a;hsp70:lin28a;Tp1:mCherry triple-transgenic fish is accompanied by reduced mCherry expression (red). Scale bar, 50 μm. B, BrdU immunofluorescence (red) shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) in the INL of uninjured retinas. Scale bar, 50 μm. C, Quantification of BrdU+ cells in Wt, hsp70:ascl1a, hsp70:lin28a, and hsp70:ascl1a;hsp70:lin28a fish treated with heat shock, ± DAPT or RO4929097; n = 3 different experiments. Error bars are SD. *p < 0.05, **p < 0.01, ***p < 0.001. D, BrdU immunofluorescence shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) throughout the INL of uninjured retinas. Scale bar, 150 μm. E, qPCR quantification of her4.1 gene expression in uninjured retinas treated with DMSO, DAPT, or RO4929097; n = 3 different experiments. Error bars are SD. ***p < 0.001.

    Article Snippet: For extended periods of heat shock, this was repeated every 6 h. To inhibit Notch signaling in fish, we immersed fish in water containing 40 μ m DAPT (Sigma-Aldrich) or RO4929097 (Cayman) prepared in DMSO and diluted 1/200 in fish water.

    Techniques: Expressing, Inhibition, Immunofluorescence, Transgenic Assay